A fluorescent antibiotic resistance marker for rapid production of transgenic rice plants

Tetsuko Ochiai-Fukuda, Naoko Takahashi-Ando, Shuichi Ohsato, Tomoko Igawa, Kaori Kadokura, Hiroshi Hamamoto, Masayoshi Nakasako, Toshiaki Kudo, Takehiko Shibata, Isamu Yamaguchi, Makoto Kimura

研究成果: Article

14 引用 (Scopus)

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Blasticidin S (BS) is an aminoacylnucleoside antibiotic used for the control of rice blast disease. To establish a new cereal transformation system, we constructed a visual marker gene designated gfbsd, encoding an enhanced green fluorescent protein (EGFP) fused to the N-terminus of BS deaminase (BSD). It was cloned into a monocot expression vector and introduced into rice (Oryza sativa L. cv. Nipponbare) calluses by microprojectile bombardment. Three to five weeks after the bombardment, multicellular clusters emitting bright-green EGFP fluorescence were obtained with 10 μg/ml BS, which is not sufficient to completely inhibit the growth of non-transformed tissues. Fluorescent sectors (approximately 2 mm in diameter) excised from the calluses regenerated into transgenic plantlets (approximately 10 cm in height) as early as 51 (average 77 ± 11) days after the bombardment. The visual antibiotic selection was more efficient and required less time than the bialaphos selection with bar. In addition, the small size (1.1 kb) of gfbsd is preferable for construction of transformation vectors. This new marker gene will make a significant contribution in molecular genetic studies of rice plants.

元の言語English
ページ(範囲)521-527
ページ数7
ジャーナルJournal of Biotechnology
122
発行部数4
DOI
出版物ステータスPublished - 2006 4 20

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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    Ochiai-Fukuda, T., Takahashi-Ando, N., Ohsato, S., Igawa, T., Kadokura, K., Hamamoto, H., Nakasako, M., Kudo, T., Shibata, T., Yamaguchi, I., & Kimura, M. (2006). A fluorescent antibiotic resistance marker for rapid production of transgenic rice plants. Journal of Biotechnology, 122(4), 521-527. https://doi.org/10.1016/j.jbiotec.2005.09.015