A genetic approach to visualization neurotechnique of multisynaptic neural pathways using plant lectin transgene

Yoshihiro Yoshihara, Takeo Mizuno, Masakiyo Nakahira, Miwa Kawasaki, Yasuyoshi Watanabe, Hiroyuki Kagamiyama, Kou ichi Jishage, Otoya Ueda, Hiroshi Suzuki, Katsuhiko Tabuchi, Kazunobu Sawamoto, Hideyuki Okano, Tetsuo Noda, Kensaku Mori

研究成果: Article査読

145 被引用数 (Scopus)

抄録

The wiring patterns among various types of neurons via specific synaptic connections are the basis of functional logic employed by the brain for information processing. This study introduces a powerful method of analyzing the neuronal connectivity patterns by delivering a tracer selectively to specific types of neurons while simultaneously transsynaptically labeling their target neurons. We developed a novel genetic approach introducing cDNA for a plant lectin, wheat germ agglutinin (WGA), as a transgene under the control of specific promoter elements. Using this method, we demonstrate three examples of visualization of specific transsynaptic neural pathways: the mouse cerebellar efferent pathways, the mouse olfactory pathways, and the Drosophila visual pathways. This strategy should greatly facilitate studies on the anatomical and functional organization of the developing and mature nervous system.

本文言語English
ページ(範囲)33-41
ページ数9
ジャーナルNeuron
22
1
DOI
出版ステータスPublished - 1999 1
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)

フィンガープリント

「A genetic approach to visualization neurotechnique of multisynaptic neural pathways using plant lectin transgene」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル