TY - JOUR
T1 - A global view of gene expression in the preimplantation mouse embryo
T2 - Genesis and Fate of the Preimplantation Embryo
AU - Tanaka, Tetsuya S.
AU - Ko, Minoru S.H.
PY - 2004/7/1
Y1 - 2004/7/1
N2 - As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. This trend was consistent with semi-quantative RT-PCR analysis of sample genes. The upregulated genes known to be involved in critical regulatory processes, included Mist1, Id2, Hd1, and Requiem; the downregulated genes included CREB-binding protein, Per3, zinc finger protein 217, Krox-25, and miwi1. Such well-characterized genes and many novel genes provide markers for early stages in development and starting materials for further functional studies.
AB - As a first step to understand preimplantation development, we performed global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray. Gene expression levels were measured four times for blastocyst and five times for morula. Student's t-test at the 5% significance level identified 428 genes upregulated and 748 downregulated in blastocyst compared to morula. This trend was consistent with semi-quantative RT-PCR analysis of sample genes. The upregulated genes known to be involved in critical regulatory processes, included Mist1, Id2, Hd1, and Requiem; the downregulated genes included CREB-binding protein, Per3, zinc finger protein 217, Krox-25, and miwi1. Such well-characterized genes and many novel genes provide markers for early stages in development and starting materials for further functional studies.
KW - Blastocyst
KW - Morula
KW - Preimplantation mouse embryos
KW - cDNA microarray
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U2 - 10.1016/j.ejogrb.2004.01.026
DO - 10.1016/j.ejogrb.2004.01.026
M3 - Conference article
C2 - 15196723
AN - SCOPUS:2942514471
VL - 115
SP - S85-S91
JO - European Journal of Obstetrics and Gynecology and Reproductive Biology
JF - European Journal of Obstetrics and Gynecology and Reproductive Biology
SN - 0028-2243
IS - SUPPL.
Y2 - 29 September 2003 through 1 October 2003
ER -