A high-throughput drug-screening system targeting ABC-transporters: An application of a microfluidic gradient generator

Y. Abe, H. Sasaki, R. Yamamoto, T. Osaki, R. Kawano, K. Kamiya, N. Miki, S. Takeuchi

研究成果: Conference contribution

1 引用 (Scopus)

抜粋

We describe a high-throughput methodology to analyze the function of ATP binding cassette transporter (ABC-transporter) by using a microfluidic device that generates a concentration gradient of substrates. Taking advantage of the basic procedure that we established for functional analysis of ABC-transporters, we here show an advanced system utilizing multi-channel microfluidic device. We applied a microfluidic gradient generator, which makes various concentrations of substrates in a single device. After incubation of ABC-transporters at 5 different substrate concentrations in a gradient generator, we succeeded in detecting the difference of substrate transport revealed by the different fluorescence intensity from ABC-transporter vesicles.

元の言語English
ホスト出版物のタイトル15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ページ885-887
ページ数3
出版物ステータスPublished - 2011 12 1
イベント15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
継続期間: 2011 10 22011 10 6

出版物シリーズ

名前15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
United States
Seattle, WA
期間11/10/211/10/6

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • これを引用

    Abe, Y., Sasaki, H., Yamamoto, R., Osaki, T., Kawano, R., Kamiya, K., Miki, N., & Takeuchi, S. (2011). A high-throughput drug-screening system targeting ABC-transporters: An application of a microfluidic gradient generator. : 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 885-887). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; 巻数 2).