A Highly Inducible System of Gene Expression by Positive Feedback Production of Glucocorticoid Receptors

Minoru S.h. Ko, Toshiya Takano

研究成果: Article査読

12 被引用数 (Scopus)

抄録

To investigate the function of a cloned gene, inducible enhancer/promoters are preferred for controlling the level of expression experimentally. Highly inducible gene expression systems with both low basal levels and enhanced maximal levels of expression are especially sought. We have developed a new expression system using a positive feedback mechanism. Glucocorticoid receptor cDNA was fused to the enhancer/promoter of mouse mammary tumor virus (MTV) to induce receptor production. Simultaneous introduction of this receptor plasmid into mouse Ltk cells augmented the expresion of the reporter gene linked with the MTV enhancer/promoter by 10- to 100-fold in an induced state, but did not significantly change the expression of that in an uninduced state. This novel system of gene expression is a good candidate for an efficient genetic switch.

本文言語English
ページ(範囲)127-133
ページ数7
ジャーナルDNA
8
2
DOI
出版ステータスPublished - 1989 3

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 遺伝学

フィンガープリント

「A Highly Inducible System of Gene Expression by Positive Feedback Production of Glucocorticoid Receptors」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル