A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles

Joe Z. Zhang, Vittavat Termglinchan, Ning Yi Shao, Ilanit Itzhaki, Chun Liu, Ning Ma, Lei Tian, Vicky Y. Wang, Alex C.Y. Chang, Hongchao Guo, Tomoya Kitani, Haodi Wu, Chi Keung Lam, Kazuki Kodo, Nazish Sayed, Helen M. Blau, Joseph C. Wu

研究成果: Article査読

64 被引用数 (Scopus)

抄録

Wu and colleagues create a double-knockin human TBX5Clover2 and NKX2-5TagRFP reporter to delineate cardiac lineages during differentiation. Molecular and functional analyses uncover that differential expression of TBX5 and NKX2-5 specifies different cardiac fate. This reporter is applicable for studying cardiac lineage determination in vitro and isolating cardiac subpopulations.

本文言語English
ページ(範囲)802-811.e5
ジャーナルCell stem cell
24
5
DOI
出版ステータスPublished - 2019 5月 2

ASJC Scopus subject areas

  • 分子医療
  • 遺伝学
  • 細胞生物学

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