抄録
This paper reports on a method for evaluating the force-length relationship of adhering human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) on the substrate using a measurement system comprising of a micromachined movable plate and a piezoresistive force probe. The cells on the plate are stretched by pushing the movable plate with the piezoresistive cantilever, which is actuated by a piezo stage. The twitch forces and the applied stretch are measured quantitatively with the piezoresistive cantilever. The results demonstrated that the twitch forces of the hiPSC-CMs increased when a stretch was applied. This evaluation method improves the understanding of the intrinsic force-length relationship of hiPSC-CMs at the cellular scale.
| 元の言語 | English |
|---|---|
| 記事番号 | 055003 |
| ジャーナル | Journal of Micromechanics and Microengineering |
| 巻 | 29 |
| 発行部数 | 5 |
| DOI | |
| 出版物ステータス | Published - 2019 3 19 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Mechanics of Materials
- Mechanical Engineering
- Electrical and Electronic Engineering
これを引用
A MEMS-based measurement system for evaluating the force-length relationship of human induced pluripotent stem cell-derived cardiomyocytes adhered on a substrate. / Matsudaira, Kenei; Takahashi, Hidetoshi; Hirayama-Shoji, Kayoko; Nguyen, Thanh Vinh; Tsukagoshi, Takuya; Shimoyama, Isao.
：: Journal of Micromechanics and Microengineering, 巻 29, 番号 5, 055003, 19.03.2019.研究成果: Article
}
TY - JOUR
T1 - A MEMS-based measurement system for evaluating the force-length relationship of human induced pluripotent stem cell-derived cardiomyocytes adhered on a substrate
AU - Matsudaira, Kenei
AU - Takahashi, Hidetoshi
AU - Hirayama-Shoji, Kayoko
AU - Nguyen, Thanh Vinh
AU - Tsukagoshi, Takuya
AU - Shimoyama, Isao
PY - 2019/3/19
Y1 - 2019/3/19
N2 - This paper reports on a method for evaluating the force-length relationship of adhering human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) on the substrate using a measurement system comprising of a micromachined movable plate and a piezoresistive force probe. The cells on the plate are stretched by pushing the movable plate with the piezoresistive cantilever, which is actuated by a piezo stage. The twitch forces and the applied stretch are measured quantitatively with the piezoresistive cantilever. The results demonstrated that the twitch forces of the hiPSC-CMs increased when a stretch was applied. This evaluation method improves the understanding of the intrinsic force-length relationship of hiPSC-CMs at the cellular scale.
AB - This paper reports on a method for evaluating the force-length relationship of adhering human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) on the substrate using a measurement system comprising of a micromachined movable plate and a piezoresistive force probe. The cells on the plate are stretched by pushing the movable plate with the piezoresistive cantilever, which is actuated by a piezo stage. The twitch forces and the applied stretch are measured quantitatively with the piezoresistive cantilever. The results demonstrated that the twitch forces of the hiPSC-CMs increased when a stretch was applied. This evaluation method improves the understanding of the intrinsic force-length relationship of hiPSC-CMs at the cellular scale.
KW - contractile force
KW - force-length relationship
KW - iPS-cell derived cardiomyocytes
KW - MEMS
KW - piezoresistive cantilever
UR - http://www.scopus.com/inward/record.url?scp=85066082750&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066082750&partnerID=8YFLogxK
U2 - 10.1088/1361-6439/ab093d
DO - 10.1088/1361-6439/ab093d
M3 - Article
AN - SCOPUS:85066082750
VL - 29
JO - Journal of Micromechanics and Microengineering
JF - Journal of Micromechanics and Microengineering
SN - 0960-1317
IS - 5
M1 - 055003
ER -