A microfabricated scaffold induces the spheroid formation of human bone marrow-derived mesenchymal progenitor cells and promotes efficient adipogenic differentiation

Yoshitaka Miyagawa, Hajime Okita, Masami Hiroyama, Ruriko Sakamoto, Maki Kobayashi, Hideki Nakajima, Yohko U. Katagiri, Junichiro Fujimoto, Jun Ichi Hata, Akihiro Umezawa, Nobutaka Kiyokawa

研究成果: Article査読

54 被引用数 (Scopus)

抄録

Here, we report the highly efficient in vitro differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (MPCs) using a novel nanotechnology-based culture plate, nanoculture plate® (NCP). The NCP contains uneven microfabrications with diameters of ∼2-3μm arranged in a honeycomb pattern on its culture surface, which is devoid of animal-derived protein sources. When human MPCs were subjected to three-dimensional (3D) culture using an NCP, they rapidly formed adhesive spheroids. We showed that adipogenic differentiation in NCP-mediated 3D cultures led to more rapid accumulation of triglycerides than that in two-dimensional cultures. During adipogenesis in 3D cultures, the rapid and intense induction of adipocyte-specific gene expressions, such as peroxisome proliferator-activated receptor γ (PPAR-γ), CCAAT-enhancer-binding protein α (C/EBP-α), adipocyte protein 2 (aP2), and adiponectin was observed. Together, these results indicate that this 3D culture system is suitable for the differentiation of human MPCs into adipogenic lineage, and could be applicable to adipose tissue engineering under xeno-free condition.

本文言語English
ページ(範囲)513-521
ページ数9
ジャーナルTissue Engineering - Part A
17
3-4
DOI
出版ステータスPublished - 2011 2月 1
外部発表はい

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 生化学
  • 生体材料
  • 生体医工学

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