A monoclonal antibody against N-terminus of α-atrial natriuretic polypeptide (α-ANP): A useful tool for preferential detection of naturally circulating ANP

M. Mukoyama, K. Nakao, T. Yamada, H. Itoh, A. Sugawara, Y. Saito, H. Arai, K. Hosoda, G. Shirakami, N. Morii, S. Shiono, H. Imura

研究成果: Article

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A monoclonal antibody directed against the N-terminus of α-atrial natriuretic polypeptide (α-ANP), named KY-ANP-II, was produced by fusion of a non-producing mouse myeloma cell line, X63-Ag8.653, with spleen cells from a BALB/c mouse immunized with synthetic α-human ANP (α-hANP) conjugated to bovine thyroglobulin. The obtained antibody showed a high affinity for α-hANP with a Ka of 6.6 x 1010 M-1. With this monoclonal antibody, a specific radioimmunoassay for α-hANP was established. The minimal detectable level of α-hANP in this radioimmunoassay was 0.8 fmol (2.5 pg)/tube, and IC50 was 8 fmol (25 pg)/tube. The radioimmunoassay recognized α-rat ANP on an equimolar basis, whereas there was no detectable cross-reactivity with α-ANP [4-28] or α-ANP [5-28]. Thus, the monoclonal antibody can detect a hormonal form of ANP, α-ANP, but not neuropeptide forms of ANP, α-ANP [4-28] and α-ANP [5-28]. These results indicate that KY-ANP-II becomes a useful tool for preferential detection of a circulating form of ANP and for investigation of the physiological and pathophysiological significance of ANP as a hormone.

元の言語English
ページ(範囲)1277-1284
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
151
発行部数3
出版物ステータスPublished - 1988 1 1
外部発表Yes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Mukoyama, M., Nakao, K., Yamada, T., Itoh, H., Sugawara, A., Saito, Y., Arai, H., Hosoda, K., Shirakami, G., Morii, N., Shiono, S., & Imura, H. (1988). A monoclonal antibody against N-terminus of α-atrial natriuretic polypeptide (α-ANP): A useful tool for preferential detection of naturally circulating ANP. Biochemical and Biophysical Research Communications, 151(3), 1277-1284.