A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity

Tanya Barrett, Tao Xie, Yulan Piao, Ora Dillon-Carter, George J. Kargul, Meng K. Lim, Francis J. Chrest, Robert Wersto, Daniel L. Rowley, Magdalena Juhaszova, Li Zhou, Marquis P. Vawter, Kevin G. Becker, Christopher Cheadle, William H. Wood, Una D. McCann, William J. Freed, Minoru S. Ko, George A. Ricaurte, David M. Donovan

研究成果: Article

29 引用 (Scopus)

抜粋

Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.

元の言語English
ページ(範囲)822-833
ページ数12
ジャーナルNeurobiology of Disease
8
発行部数5
DOI
出版物ステータスPublished - 2001 1 1
外部発表Yes

ASJC Scopus subject areas

  • Neurology

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    Barrett, T., Xie, T., Piao, Y., Dillon-Carter, O., Kargul, G. J., Lim, M. K., Chrest, F. J., Wersto, R., Rowley, D. L., Juhaszova, M., Zhou, L., Vawter, M. P., Becker, K. G., Cheadle, C., Wood, W. H., McCann, U. D., Freed, W. J., Ko, M. S., Ricaurte, G. A., & Donovan, D. M. (2001). A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity. Neurobiology of Disease, 8(5), 822-833. https://doi.org/10.1006/nbdi.2001.0423