@article{98ef0fbc568a4216a3e9960b775790b5,
title = "A murine dopamine neuron-specific cDNA library and microarray: Increased COX1 expression during methamphetamine neurotoxicity",
abstract = "Due to brain tissue heterogeneity, the molecular genetic profile of any neurotransmitter-specific neuronal subtype is unknown. The purpose of this study was to purify a population of dopamine neurons, construct a cDNA library, and generate an initial gene expression profile and a microarray representative of dopamine neuron transcripts. Ventral mesencephalic dopamine neurons were purified by fluorescent-activated cell sorting from embryonic day 13.5 transgenic mice harboring a 4.5-kb rat tyrosine hydroxylase promoter-lacZ fusion. Nine-hundred sixty dopamine neuron cDNA clones were sequenced and arrayed for use in studies of gene expression changes during methamphetamine neurotoxicity. A neurotoxic dose of methamphetamine produced a greater than twofold up-regulation of the mitochondrial cytochrome c oxidase polypeptide I transcript from adult mouse substantia nigra at 12 h posttreatment. This is the first work to describe a gene expression profile for a neuronal subtype and to identify gene expression changes during methamphetamine neurotoxicity.",
keywords = "Development, Dopamine, Gene expression profile, Methamphetamine, cDNA library, cDNA microarray",
author = "Tanya Barrett and Tao Xie and Yulan Piao and Ora Dillon-Carter and Kargul, {George J.} and Lim, {Meng K.} and Chrest, {Francis J.} and Robert Wersto and Rowley, {Daniel L.} and Magdalena Juhaszova and Li Zhou and Vawter, {Marquis P.} and Becker, {Kevin G.} and Christopher Cheadle and Wood, {William H.} and McCann, {Una D.} and Freed, {William J.} and Ko, {Minoru S.} and Ricaurte, {George A.} and Donovan, {David M.}",
note = "Funding Information: This research was supported, in part, by grants from the National Institute on Drug Abuse (Supported by PHS Grants DA13790 and DA00206). We are indebted to Wayne French, Nancy Williams, Renee Kahn, Virginia Miller, and Donna Ritter of the Animal Resources Section, NIA, for their support in the maintenance of the mouse colony and production of timed pregnant female mice. We also thank Tom Wynn of the Photography and Arts Section, NIA, for his professional and timely help with the manuscript.",
year = "2001",
doi = "10.1006/nbdi.2001.0423",
language = "English",
volume = "8",
pages = "822--833",
journal = "Neurobiology of Disease",
issn = "0969-9961",
publisher = "Academic Press Inc.",
number = "5",
}