A new member of the tripartite multidrug efflux pumps, MexVW-OprM, in Pseudomonas aeruginosa

Yang Li, Takehiko Mima, Yukiko Komori, Yuji Morita, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

研究成果: Article

66 引用 (Scopus)

抄録

Objectives: Multidrug efflux pumps are thought to be involved in mediating multidrug resistance in Pseudomonas aeruginosa. Here we aim to characterize hitherto uncharacterized multidrug efflux pumps from P. aeruginosa. Materials and methods: We isolated a mutant, YM442, which showed elevated resistance to several antimicrobial agents from P. aeruginosa YM44 lacking four major multidrug efflux pumps, MexAB, MexCD-OprJ, MexEF-OprN and MexXY. We cloned genes responsible for the resistance from chromosomal DNA of YM442 using YM44 as host. Results: We designated the genes mexVW. Introduction of a recombinant plasmid pTAJ2 carrying the mexVW into YM44 cells conferred resistance to fluoroquinolones, tetracycline, chloramphenicol, erythromycin, ethidium bromide and acriflavine. Elevated ethidium bromide extrusion was observed with cells of YM442 and of YM44/pTAJ2. An outer membrane protein OprM was able to cooperate with MexVW. Elevated expression of the mexV gene was observed with YM442 compared with YM44. Conclusions: MexV (membrane fusion protein)-MexW (RND-type membrane protein)-OprM is a tripartite multidrug efflux pump. It is suggested that other outer membrane component(s) could cooperate with MexVW.

元の言語English
ページ(範囲)572-575
ページ数4
ジャーナルJournal of Antimicrobial Chemotherapy
52
発行部数4
DOI
出版物ステータスPublished - 2003 10 1
外部発表Yes

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Pseudomonas aeruginosa
Ethidium
Membrane Proteins
Membrane Fusion Proteins
Acriflavine
Fluoroquinolones
Multiple Drug Resistance
Chloramphenicol
Erythromycin
Anti-Infective Agents
Tetracycline
Genes
Plasmids
Gene Expression
Membranes
DNA

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

これを引用

Li, Y., Mima, T., Komori, Y., Morita, Y., Kuroda, T., Mizushima, T., & Tsuchiya, T. (2003). A new member of the tripartite multidrug efflux pumps, MexVW-OprM, in Pseudomonas aeruginosa. Journal of Antimicrobial Chemotherapy, 52(4), 572-575. https://doi.org/10.1093/jac/dkg390

A new member of the tripartite multidrug efflux pumps, MexVW-OprM, in Pseudomonas aeruginosa. / Li, Yang; Mima, Takehiko; Komori, Yukiko; Morita, Yuji; Kuroda, Teruo; Mizushima, Tohru; Tsuchiya, Tomofusa.

:: Journal of Antimicrobial Chemotherapy, 巻 52, 番号 4, 01.10.2003, p. 572-575.

研究成果: Article

Li, Y, Mima, T, Komori, Y, Morita, Y, Kuroda, T, Mizushima, T & Tsuchiya, T 2003, 'A new member of the tripartite multidrug efflux pumps, MexVW-OprM, in Pseudomonas aeruginosa', Journal of Antimicrobial Chemotherapy, 巻. 52, 番号 4, pp. 572-575. https://doi.org/10.1093/jac/dkg390
Li, Yang ; Mima, Takehiko ; Komori, Yukiko ; Morita, Yuji ; Kuroda, Teruo ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / A new member of the tripartite multidrug efflux pumps, MexVW-OprM, in Pseudomonas aeruginosa. :: Journal of Antimicrobial Chemotherapy. 2003 ; 巻 52, 番号 4. pp. 572-575.
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abstract = "Objectives: Multidrug efflux pumps are thought to be involved in mediating multidrug resistance in Pseudomonas aeruginosa. Here we aim to characterize hitherto uncharacterized multidrug efflux pumps from P. aeruginosa. Materials and methods: We isolated a mutant, YM442, which showed elevated resistance to several antimicrobial agents from P. aeruginosa YM44 lacking four major multidrug efflux pumps, MexAB, MexCD-OprJ, MexEF-OprN and MexXY. We cloned genes responsible for the resistance from chromosomal DNA of YM442 using YM44 as host. Results: We designated the genes mexVW. Introduction of a recombinant plasmid pTAJ2 carrying the mexVW into YM44 cells conferred resistance to fluoroquinolones, tetracycline, chloramphenicol, erythromycin, ethidium bromide and acriflavine. Elevated ethidium bromide extrusion was observed with cells of YM442 and of YM44/pTAJ2. An outer membrane protein OprM was able to cooperate with MexVW. Elevated expression of the mexV gene was observed with YM442 compared with YM44. Conclusions: MexV (membrane fusion protein)-MexW (RND-type membrane protein)-OprM is a tripartite multidrug efflux pump. It is suggested that other outer membrane component(s) could cooperate with MexVW.",
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AU - Li, Yang

AU - Mima, Takehiko

AU - Komori, Yukiko

AU - Morita, Yuji

AU - Kuroda, Teruo

AU - Mizushima, Tohru

AU - Tsuchiya, Tomofusa

PY - 2003/10/1

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N2 - Objectives: Multidrug efflux pumps are thought to be involved in mediating multidrug resistance in Pseudomonas aeruginosa. Here we aim to characterize hitherto uncharacterized multidrug efflux pumps from P. aeruginosa. Materials and methods: We isolated a mutant, YM442, which showed elevated resistance to several antimicrobial agents from P. aeruginosa YM44 lacking four major multidrug efflux pumps, MexAB, MexCD-OprJ, MexEF-OprN and MexXY. We cloned genes responsible for the resistance from chromosomal DNA of YM442 using YM44 as host. Results: We designated the genes mexVW. Introduction of a recombinant plasmid pTAJ2 carrying the mexVW into YM44 cells conferred resistance to fluoroquinolones, tetracycline, chloramphenicol, erythromycin, ethidium bromide and acriflavine. Elevated ethidium bromide extrusion was observed with cells of YM442 and of YM44/pTAJ2. An outer membrane protein OprM was able to cooperate with MexVW. Elevated expression of the mexV gene was observed with YM442 compared with YM44. Conclusions: MexV (membrane fusion protein)-MexW (RND-type membrane protein)-OprM is a tripartite multidrug efflux pump. It is suggested that other outer membrane component(s) could cooperate with MexVW.

AB - Objectives: Multidrug efflux pumps are thought to be involved in mediating multidrug resistance in Pseudomonas aeruginosa. Here we aim to characterize hitherto uncharacterized multidrug efflux pumps from P. aeruginosa. Materials and methods: We isolated a mutant, YM442, which showed elevated resistance to several antimicrobial agents from P. aeruginosa YM44 lacking four major multidrug efflux pumps, MexAB, MexCD-OprJ, MexEF-OprN and MexXY. We cloned genes responsible for the resistance from chromosomal DNA of YM442 using YM44 as host. Results: We designated the genes mexVW. Introduction of a recombinant plasmid pTAJ2 carrying the mexVW into YM44 cells conferred resistance to fluoroquinolones, tetracycline, chloramphenicol, erythromycin, ethidium bromide and acriflavine. Elevated ethidium bromide extrusion was observed with cells of YM442 and of YM44/pTAJ2. An outer membrane protein OprM was able to cooperate with MexVW. Elevated expression of the mexV gene was observed with YM442 compared with YM44. Conclusions: MexV (membrane fusion protein)-MexW (RND-type membrane protein)-OprM is a tripartite multidrug efflux pump. It is suggested that other outer membrane component(s) could cooperate with MexVW.

KW - MexVW

KW - Multidrug efflux pump

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