A new method for manufacturing cardiac cell sheets using fibrin-coated dishes and its electrophysiological studies by optical mapping

Yuji Itabashi, Shunichiro Miyoshi, Haruko Kawaguchi, Shinsuke Yuasa, Kojiro Tanimoto, Akira Furuta, Tatsuya Shimizu, Teruo Okano, Keiichi Fukuda, Satoshi Ogawa

研究成果: Article

64 引用 (Scopus)

抄録

We developed a novel simple method for making functional myocardial cell sheets that may be used as transplants. Polymerized human fibrin-coated dishes were prepared with fibrinogen monomers mixed with thrombin. Neonatal rat cardiomyocytes cultured on these dishes formed myocardial cell sheets within 4 days. These cell sheets were easily dissociated intact from the polymerized fibrin layer, because the fibrin had been digested by intrinsic protease. Two overlaid myocardial cell sheets exhibited synchronized spontaneous beating and captured artificial pacing. Optical mapping confirmed that the conduction of the action potential between two partially overlaid myocardial cell sheets was established, and the action potential propagated across the junction without any delay. Transplanted three-layered myocardial cell sheets exhibited strong spontaneous beating and showed well-differentiated striations and an increase in cell size. This simple method of cell sheet engineering may also be applicable for various other cell types.

元の言語English
ページ(範囲)95-103
ページ数9
ジャーナルArtificial Organs
29
発行部数2
DOI
出版物ステータスPublished - 2005 2

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Transplants
Fibrin
Rats
Monomers
Thrombin
Fibrinogen
Peptide Hydrolases
Action Potentials
Cell Engineering
Cell Size
Cardiac Myocytes

ASJC Scopus subject areas

  • Biophysics

これを引用

A new method for manufacturing cardiac cell sheets using fibrin-coated dishes and its electrophysiological studies by optical mapping. / Itabashi, Yuji; Miyoshi, Shunichiro; Kawaguchi, Haruko; Yuasa, Shinsuke; Tanimoto, Kojiro; Furuta, Akira; Shimizu, Tatsuya; Okano, Teruo; Fukuda, Keiichi; Ogawa, Satoshi.

:: Artificial Organs, 巻 29, 番号 2, 02.2005, p. 95-103.

研究成果: Article

Itabashi, Yuji ; Miyoshi, Shunichiro ; Kawaguchi, Haruko ; Yuasa, Shinsuke ; Tanimoto, Kojiro ; Furuta, Akira ; Shimizu, Tatsuya ; Okano, Teruo ; Fukuda, Keiichi ; Ogawa, Satoshi. / A new method for manufacturing cardiac cell sheets using fibrin-coated dishes and its electrophysiological studies by optical mapping. :: Artificial Organs. 2005 ; 巻 29, 番号 2. pp. 95-103.
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abstract = "We developed a novel simple method for making functional myocardial cell sheets that may be used as transplants. Polymerized human fibrin-coated dishes were prepared with fibrinogen monomers mixed with thrombin. Neonatal rat cardiomyocytes cultured on these dishes formed myocardial cell sheets within 4 days. These cell sheets were easily dissociated intact from the polymerized fibrin layer, because the fibrin had been digested by intrinsic protease. Two overlaid myocardial cell sheets exhibited synchronized spontaneous beating and captured artificial pacing. Optical mapping confirmed that the conduction of the action potential between two partially overlaid myocardial cell sheets was established, and the action potential propagated across the junction without any delay. Transplanted three-layered myocardial cell sheets exhibited strong spontaneous beating and showed well-differentiated striations and an increase in cell size. This simple method of cell sheet engineering may also be applicable for various other cell types.",
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AU - Itabashi, Yuji

AU - Miyoshi, Shunichiro

AU - Kawaguchi, Haruko

AU - Yuasa, Shinsuke

AU - Tanimoto, Kojiro

AU - Furuta, Akira

AU - Shimizu, Tatsuya

AU - Okano, Teruo

AU - Fukuda, Keiichi

AU - Ogawa, Satoshi

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N2 - We developed a novel simple method for making functional myocardial cell sheets that may be used as transplants. Polymerized human fibrin-coated dishes were prepared with fibrinogen monomers mixed with thrombin. Neonatal rat cardiomyocytes cultured on these dishes formed myocardial cell sheets within 4 days. These cell sheets were easily dissociated intact from the polymerized fibrin layer, because the fibrin had been digested by intrinsic protease. Two overlaid myocardial cell sheets exhibited synchronized spontaneous beating and captured artificial pacing. Optical mapping confirmed that the conduction of the action potential between two partially overlaid myocardial cell sheets was established, and the action potential propagated across the junction without any delay. Transplanted three-layered myocardial cell sheets exhibited strong spontaneous beating and showed well-differentiated striations and an increase in cell size. This simple method of cell sheet engineering may also be applicable for various other cell types.

AB - We developed a novel simple method for making functional myocardial cell sheets that may be used as transplants. Polymerized human fibrin-coated dishes were prepared with fibrinogen monomers mixed with thrombin. Neonatal rat cardiomyocytes cultured on these dishes formed myocardial cell sheets within 4 days. These cell sheets were easily dissociated intact from the polymerized fibrin layer, because the fibrin had been digested by intrinsic protease. Two overlaid myocardial cell sheets exhibited synchronized spontaneous beating and captured artificial pacing. Optical mapping confirmed that the conduction of the action potential between two partially overlaid myocardial cell sheets was established, and the action potential propagated across the junction without any delay. Transplanted three-layered myocardial cell sheets exhibited strong spontaneous beating and showed well-differentiated striations and an increase in cell size. This simple method of cell sheet engineering may also be applicable for various other cell types.

KW - Cardiomyocyte

KW - Cell sheet

KW - Electrical connection

KW - Optical mapping

KW - Polymerized fibrin

KW - Tissue engineering

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