A new predictive factor for hepatocellular carcinoma based on two-dimensional electrophoresis of genomic DNA

Osamu Itano, Masakazu Ueda, Kiyoshi Kikuchi, Motohide Shimazu, Yuko Kitagawa, Kouichi Aiura, Masaki Kitajima

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Molecular genetic analyses have clarified that accumulation of genomic changes provides important steps in carcinogenesis and have identified a number of valuable genetic markers for certain cancers. To date, however, no prognostic markers have been identified for hepatocellular carcinoma (HCC). In this study, we used restriction landmark genomic scanning (RLGS), a new high-speed screening method for multiple genomic changes, to detect unknown genetic alterations in HCC. Thirty-one HCC samples and their normal counterparts were examined by RLGS. Eight spot changes were common in several cases, and all were seen only on the HCC profile. Five of these spots were detected in more than 12 of 31 cases (38.7%). Viral infection had no influence on changes in the RLGS spots. The disease-free survival rate for patients with ≥ 16 changed RLGS spots was significantly lower than that for patients with fewer changed RLGS spots( ≤ 15 spots) (P < 0.001). In multivariate analysis, the number of changed spots was proven to retain an independent prognostic value (relative risk 1.095: P = 0.0031). These results suggest that the number of changed RLGS spots may be a useful biological marker for recurrence of HCC.

本文言語English
ページ(範囲)1676-1683
ページ数8
ジャーナルOncogene
19
13
DOI
出版ステータスPublished - 2000 3 23

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学
  • 癌研究

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