A novel mouse model for Down syndrome that harbor a single copy of human artificial chromosome (HAC) carrying a limited number of genes from human chromosome 21.

Kenichi Miyamoto, Nobutaka Suzuki, Kosuke Sakai, Shuichi Asakawa, Tsuneko Okazaki, Jun Kudoh, Masashi Ikeno, Nobuyoshi Shimizu

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Down syndrome (DS), also known as Trisomy 21, is the most common chromosome aneuploidy in live-born children and displays a complicated symptom. To date, several kinds of mouse models have been generated to understand the molecular pathology of DS, yet the gene dosage effects and gene(s)-phenotype(s) correlation are not well understood. In this study, we established a novel method to generate a partial trisomy mice using the mouse ES cells that harbor a single copy of human artificial chromosome (HAC), into which a small human DNA segment containing human chromosome 21 genes cloned in a bacterial artificial chromosome (BAC) was recombined. The produced mice were found to maintain the HAC carrying human genes as a mini-chromosome, hence termed as a Trans-Mini-Chromosomal (TMC) mouse, and HAC was transmitted for more than twenty generations independent from endogenous mouse chromosomes. The three human transgenes including cystathionine β-synthase, U2 auxiliary factor and crystalline alpha A were expressed in several mouse tissues with various expression levels relative to mouse endogenous genes. The novel system is applicable to any of human and/or mouse BAC clones. Thus, the TMC mouse carrying a HAC with a limited number of genes would provide a novel tool for studying gene dosage effects involved in the DS molecular pathogenesis and the gene(s)-phenotype(s) correlation.

本文言語English
ページ(範囲)317-329
ページ数13
ジャーナルTransgenic research
23
2
DOI
出版ステータスPublished - 2014 4

ASJC Scopus subject areas

  • バイオテクノロジー
  • 動物科学および動物学
  • 遺伝学
  • 農業および作物学

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