A novel yeast cell-based screen identifies flavone as a tankyrase inhibitor

Yoko Yashiroda, Reika Okamoto, Kaori Hatsugai, Yasushi Takemoto, Naoki Goshima, Tamio Saito, Makiko Hamamoto, Yoshikazu Sugimoto, Hiroyuki Osada, Hiroyuki Seimiya, Minoru Yoshida

研究成果: Article

30 被引用数 (Scopus)

抄録

The telomere-associated protein tankyrase 1 is a poly(ADP-ribose) polymerase and is considered to be a promising target for cancer therapy, especially for BRCA-associated cancers. However, an efficient assay system for inhibitor screening has not been established, mainly due to the difficulty of efficient preparation of the enzyme and its substrate. Here, we report a cell-based assay system for detecting inhibitory activity against tankyrase 1. We found that overexpression of the human tankyrase 1 gene causes a growth defect in the fission yeast Schizosaccharomyces pombe. Chemicals that restore the growth defect phenotype can be identified as potential tankyrase 1 inhibitors. We performed a high-throughput screen using this system, and identified flavone as a compound that restores the growth of yeast cells overexpressing tankyrase 1. Indeed, flavone inhibited poly(ADP-ribosyl)ation of proteins caused by overexpression of tankyrase 1 in yeast cells. This system allows rapid identification of inhibitory activity against tankyrase 1 and is amenable to high-throughput screening using robotics.

本文言語English
ページ(範囲)569-573
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
394
3
DOI
出版ステータスPublished - 2010 4 9

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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