TY - JOUR
T1 - A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 7 (matrilysin) using monoclonal antibodies
AU - Ohuchia, Eiko
AU - Azumano, Isao
AU - Yoshida, Shin'ichi
AU - Iwata, Kazushi
AU - Okada, Yasunori
PY - 1996/1/31
Y1 - 1996/1/31
N2 - A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 7 (MMP-7, matrilysin, EC 3.4.24.23) was established with a pair of monoclonal antibodies prepared against the zymogen of MMP-7 (proMMP-7) purified from CaR-1 human rectal carcinoma cells and against an oligopeptide corresponding to the C-terminal domain of human proMMP-7. ProMMP-7 in samples was allowed to simultaneously react with both solid-phase and peroxidase-labeled antibodies. Sensitivity of the EIA system in the presence of EDTA was 0.05 μg/l (1.5 pg/assay) and linearity was obtained between 0.156-10 μg/l (4.68-300 pg/assay). ProMMP-7 levels in human sera from healthy subjects were shown to be in the range of 10.7 ± 18.8 μg/l. Gel filtration analyses indicated that proMMP-7 makes polymers after treatment with EDTA and suggested that proMMP-7 exists as polymers in normal human sera.
AB - A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 7 (MMP-7, matrilysin, EC 3.4.24.23) was established with a pair of monoclonal antibodies prepared against the zymogen of MMP-7 (proMMP-7) purified from CaR-1 human rectal carcinoma cells and against an oligopeptide corresponding to the C-terminal domain of human proMMP-7. ProMMP-7 in samples was allowed to simultaneously react with both solid-phase and peroxidase-labeled antibodies. Sensitivity of the EIA system in the presence of EDTA was 0.05 μg/l (1.5 pg/assay) and linearity was obtained between 0.156-10 μg/l (4.68-300 pg/assay). ProMMP-7 levels in human sera from healthy subjects were shown to be in the range of 10.7 ± 18.8 μg/l. Gel filtration analyses indicated that proMMP-7 makes polymers after treatment with EDTA and suggested that proMMP-7 exists as polymers in normal human sera.
KW - Matrix metalloproteinase 7
KW - Monoclonal antibody
KW - Sandwich enzyme immunoassay
KW - Tissue inhibitor of metalloproteinases
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U2 - 10.1016/0009-8981(95)06199-1
DO - 10.1016/0009-8981(95)06199-1
M3 - Article
C2 - 8714436
AN - SCOPUS:0030021904
SN - 0009-8981
VL - 244
SP - 181
EP - 198
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 2
ER -
