A portable lipid bilayer system for environmental sensing with a transmembrane protein

Ryuji Kawano, Yutaro Tsuji, Koki Kamiya, Taiga Kodama, Toshihisa Osaki, Norihisa Miki, Shoji Takeuchi

研究成果: Article査読

35 被引用数 (Scopus)

抄録

This paper describes a portable measurement system for current signals of an ion channel that is composed of a planar lipid bilayer. A stable and reproducible lipid bilayer is formed in outdoor environments by using a droplet contact method with a micropipette. Using this system, we demonstrated that the single-channel recording of a transmembrane protein (alpha-hemolysin) was achieved in the field at a high-altitude (∼3623 m). This system would be broadly applicable for obtaining environmental measurements using membrane proteins as a highly sensitive sensor.

本文言語English
論文番号e102427
ジャーナルPloS one
9
7
DOI
出版ステータスPublished - 2014 7 29

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

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