TY - JOUR
T1 - A possible inhibitory role of sialic acid on muc1 in peritoneal dissemination of clear cell-type ovarian cancer cells
AU - Tamada, Yutaka
AU - Nomura, Hiroyuki
AU - Aoki, Daisuke
AU - Irimura, Tatsuro
N1 - Funding Information:
Funding: This research was funded by Grants-in-Aid from the Japan Society for the Promotion of Science (grant number 25293011 to T.I.). This work was also funded by the Fukuzawa Memorial Keio Academic Affairs Promotion Fund and by Grants-in-Aid from the Japan Ministry of Education, Culture, Sports, Science and Technology (grant number 16790968 to Y.T.).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/10/1
Y1 - 2021/10/1
N2 - The role of sialic acids on MUC1 in peritoneal dissemination of ovarian cancer cells was investigated. A human ovarian carcinoma cell line, ES-2, was transfected with full-length MUC1 containing 22 or 42 tandem repeats. These transfectants were less adherent to monolayers of patient-derived mesothelial cells than ES-2/mock transfectants. When these cells were inoculated into the abdominal cavity of female nude mice, mice that had received the transfectants showed better survival. When the transfectants were mixed with sialidase and injected, the survival was poorer, whereas when they were mixed with N-acetyl-2,3-dehydro-2-deoxyneuraminic acid, a sialidase inhibitor, the survival was significantly prolonged. These behaviors, concerned with peritoneal implantation and dissemination observed in vitro and in vivo, were dependent on the expression of MUC1. Therefore, sialic acid linked to MUC1 in the form, at least in part, of sialyl-T, as shown to be recognized by monoclonal antibody MY.1E12, is responsible for the suppression of adhesion of these cells to mesothelial cells and the suppression of peritoneal implantation and dissemination.
AB - The role of sialic acids on MUC1 in peritoneal dissemination of ovarian cancer cells was investigated. A human ovarian carcinoma cell line, ES-2, was transfected with full-length MUC1 containing 22 or 42 tandem repeats. These transfectants were less adherent to monolayers of patient-derived mesothelial cells than ES-2/mock transfectants. When these cells were inoculated into the abdominal cavity of female nude mice, mice that had received the transfectants showed better survival. When the transfectants were mixed with sialidase and injected, the survival was poorer, whereas when they were mixed with N-acetyl-2,3-dehydro-2-deoxyneuraminic acid, a sialidase inhibitor, the survival was significantly prolonged. These behaviors, concerned with peritoneal implantation and dissemination observed in vitro and in vivo, were dependent on the expression of MUC1. Therefore, sialic acid linked to MUC1 in the form, at least in part, of sialyl-T, as shown to be recognized by monoclonal antibody MY.1E12, is responsible for the suppression of adhesion of these cells to mesothelial cells and the suppression of peritoneal implantation and dissemination.
KW - Mucins
KW - Neuraminidase
KW - Ovarian neoplasms
KW - Peritoneal dissemination
KW - Sialic acid
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U2 - 10.3390/molecules26195962
DO - 10.3390/molecules26195962
M3 - Article
C2 - 34641504
AN - SCOPUS:85116512598
SN - 1420-3049
VL - 26
JO - Molecules
JF - Molecules
IS - 19
M1 - 5962
ER -
