A potential link between transgene silencing and poly(A) tails

Mikiko C. Siomi, Hiroko Tsukumo, Akira Ishizuka, Tomoko Nagami, Haruhiko Siomi

研究成果: Article

13 引用 (Scopus)

抜粋

Argonaute proteins function in gene silencing induced by double-stranded RNA (dsRNA) in various organisms. In Drosophila, the Argonaute proteins AGO1 and AGO2 have been implicated in post-transcriptional gene-silencing (PTGS)/RNA interference (RNAi). In this study, we found that AGO1 and AGO2 depletion caused the accumulation of multicopied enhanced green fluorescence protein (EGFP) transgene transcripts in Drosophila S2 cells. Depletion of AGO1, the essential factor for miRNA biogenesis, led to an increased transcriptional rate of the transgenes. In contrast, depletion of AGO2, the essential factor for siRNA-directed RNAi, resulted in EGFP mRNA stabilization with concomitant shortening of the EGFP mRNA poly(A) tail. Our findings suggest that AGO1 and AGO2 mediate multicopied transgene silencing by different mechanisms. Intriguingly, Dicer2 depletion phenocopies AGO2 depletion, with an increase in EGFP protein levels and shortening of the EGFP mRNA poly(A) tail. The possibility that AGO2 and Dicer2 involve, at least in part, poly(A) length maintenance of transgene mRNA suggests a potentially important link between transgene silencing and poly(A) tails.

元の言語English
ページ(範囲)1004-1011
ページ数8
ジャーナルRNA
11
発行部数7
DOI
出版物ステータスPublished - 2005 7

ASJC Scopus subject areas

  • Molecular Biology

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  • これを引用

    Siomi, M. C., Tsukumo, H., Ishizuka, A., Nagami, T., & Siomi, H. (2005). A potential link between transgene silencing and poly(A) tails. RNA, 11(7), 1004-1011. https://doi.org/10.1261/rna.2280105