A practical device for pinpoint delivery of molecules into multiple neurons in culture

Chikako Hara, Kiyohiko Tateyama, Naoki Akamatsu, Hiroyuki Imabayashi, Koichi Karaki, Nobuo Nomura, Hideyuki Okano, Atsushi Miyawaki

研究成果: Article査読

15 被引用数 (Scopus)

抄録

We have developed a device for pinpoint delivery of chemicals, proteins, and nucleic acids into cultured cells. The principle underlying the technique is the flow of molecules from the culture medium into cells through a rupture in the plasma membrane made by a needle puncture. DNA transfection is achieved by stabbing the needle tip into the nucleus. The CellBee device can be attached to any inverted microscope, and molecular delivery can be coupled with conventional live cell imaging. Because the position of the needle relative to the targeted cultured cells is computer-controlled, efficient delivery of molecules such as rhodamine into as many as 100 HeLa cells can be completed in 10 min. Moreover, specific target cells within a single dish can be transfected with multiple DNA constructs by simple changes of culture medium containing different plasmids. In addition, the nano-sized needle tip enables gentle molecular delivery, minimizing cell damage. This method permits DNA transfection into specific hippocampal neurons without disturbing neuronal circuitry established in culture.

本文言語English
ページ(範囲)229-237
ページ数9
ジャーナルBrain Cell Biology
35
4-6
DOI
出版ステータスPublished - 2006 12月

ASJC Scopus subject areas

  • 細胞および分子神経科学
  • 細胞生物学

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