A quantitative matrigel assay for assessing repopulating capacity of prostate stem cells

Masaki Azuma, Atsushi Hirao, Keiyo Takubo, Isao Hamaguchi, Tadaichi Kitamura, Toshio Suda

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Homeostasis of prostate tissue is maintained by stem cells, although such cells have not been well characterized. Here, we report establishment of such a method using matrigel. Matrigel containing a single-cell suspension from adult prostatic cells was subcutaneously grafted into the flank of nude mice. Prostatic duct-like structures derived from donor tissue were observed in the gel 2 weeks after transplantation. Luminal and basal cells observed in the gel expressed several markers characteristic of prostatic and/or epithelial cells. When a mixture with both EGFP-positive and negative prostate cells was transplanted, prostatic ducts consisted of either EGFP-positive or negative cells and chimeric patterns were rarely observed, suggesting that ducts were reconstituted from a single cell. Stem cell number and function were also evaluated by competition with control cells. Overall this method revealed that cells localized in the proximal portion in prostate ducts had higher reconstitution capacity than those in the distal portion. We conclude that prostate stem/progenitor cells exist and that our method is applicable to analysis of prostate stem cells, epithelial mesenchyme interactions, and prostate cancer stem cells.

本文言語English
ページ(範囲)1164-1170
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
338
2
DOI
出版ステータスPublished - 2005 12 16

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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