A region of basic amino-acid cluster in HIV-1 Tat protein is essential for Trans-acting activity and nucleolar localization

Sei Ichi Endo, Satoshi Kubota, Haruhiko Siomi, Akio Adachi, Stephen Oroszlan, Masatoshi Maki, Masakazu Hatanaka

研究成果: Article

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The trans-acting factor of human immunodeficiency virus (HIV), Tat, has a basic amino-acid cluster that is highly conserved among different HIV isolates. We have examined the effects of mutations in the basic region of Tat on its trans-acting activity and cellular localization. Introduction of a stop codon immediately preceding the basic region abolished the activity, while the truncated mutant with the basic region retained some activity. The basic region of Tat was replaceable with that of Rev (another trans-acting factor of HIV) but not with that of adenovirus Ela nor cellular enzyme. The result of immunofluorescence analysis revealed a correlation between the nuclear, especially nucleolar, accumulation and the activities of mutant Tat proteins.

元の言語English
ページ(範囲)99-110
ページ数12
ジャーナルVirus Genes
3
発行部数2
DOI
出版物ステータスPublished - 1989 11 1
外部発表Yes

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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