A role for hematopoietic stem cells in promoting angiogenesis

Nobuyuki Takakura, Toshio Watanabe, Souichi Suenobu, Yoshihiro Yamada, Tetsuo Noda, Yoshiaki Ito, Masanobu Satake, Toshio Suda

研究成果: Article査読

468 被引用数 (Scopus)

抄録

Angiogenesis is an important event for embryonic organogenesis as well as for tissue repair in the adult. Here, we show that hematopoietic stem cells (HSCs) play important roles for angiogenesis during embryogenesis. To investigate the role of HSCs in endothelial cell (EC) development, we analyzed AML1-deficient embryos, which lack definitive hematopoiesis. These embryos showed defective angiogenesis in the head and pericardium. Para-aortic splanchnopleural (P-Sp) explant cultures on stromal cells (P-Sp culture) did not generate definitive hematopoietic cells and showed defective angiogenesis in the AML1 null embryo. Disrupted angiogenesis in P-Sp cultures from AML1 null embryos was rescued by addition of HSCs or angiopoietin-1 (Ang1). HSCs, which express Ang1, directly promoted migration of ECs in vivo and in vitro. These results indicate that HSCs are critical for angiogenesis.

本文言語English
ページ(範囲)199-209
ページ数11
ジャーナルCell
102
2
DOI
出版ステータスPublished - 2000 7月 21
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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