A single molecular multianalyte fluorescent probe and its application to imaging intracellular multianalyte dynamics

The quantitative analysis of a single molecular multianalyte fluorescent probes that is used for intercellular multianalyte imaging is discussed. The correlation of the intracellular Ca ₂₊ and Mg ₂₊ ions were done with simultaneous imaging of both cations using a novel single molecular multianalyte sensor. The molecular design of semiselectivity and discrimination of the analytes are required for the optical discrimination of the analytes. The biding characteristics of fluorescent probes are compared based on their dissociation constant. The optical properties of KCM-1 were measured for different cation concentrations, and the response was compared to the mathematical simulation that assumes two chemical equilibriums. The concept of molecular multianalyte sensors can be extended to other molecular sensors such as chromoionophores, green fluorescent protein (GFP) based sensors and contrast agents.