抄録
A two-step sandwich enzyme immunoassay (EIA) system for the precursor of rabbit MMP-3 (r-proMMP-3) using monoclonal antibodies has been established to quantify the proMMP-3 levels in t he rabbit body fluids. The EIA system takes 150 min for the reactions (120 min for the first and second immunoreactions and 30 min for color reaction at 20°C). Sensitivity for rabbit proMMP-3 was 4.7 ng/ml (140 pg/assay) and linearity was obtained from 17 1,067 ng/ml (0.5 32 ng/assay). The EIA system recognizes only proMMP-3, but not its reactive forms. The levels of proMMP-3 (111 ± 9.3 ng/ml) in the synovial fluids from rabbit osteoarthritic models were higher than those from the normal control animals.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 183-190 |
| ページ数 | 8 |
| ジャーナル | Connective Tissue |
| 巻 | 27 |
| 号 | 3-4 |
| 出版ステータス | Published - 1995 12 1 |
ASJC Scopus subject areas
- リウマチ学