Aberrant fatty acid-binding protein-7 gene expression in cutaneous malignant melanoma

Yasufumi Goto, Kazuo Koyanagi, Norihiko Narita, Yutaka Kawakami, Minoru Takata, Aya Uchiyama, Linhda Nguyen, Tung Nguyen, Xing Ye, Donald L. Morton, Dave S.B. Hoon

研究成果: Article査読

27 被引用数 (Scopus)

抄録

Fatty acid-binding protein-7 (FABP7) has been shown to be expressed in cutaneous melanoma; however, its role in tumor progression is unclear. Expression of FABP7 was assessed during melanoma progression through assessment of various clinicopathology stages of primary tumor progression and metastasis. FABP7 mRNA was highly expressed in 60 of 87 (69%) primary melanomas, compared with significant (P0.0001) reduction in 13 of 68 (19%) metastatic melanomas. Analysis of 37 paired primary and metastatic melanomas by immunohistochemistry with anti-FABP7 Ab showed 73 and 27% positivity, respectively (P0.001). FABP7 detection of metastatic tissues was inversely correlated with relapse-free (P0.0001) and overall (P0.0001) survival. To examine FABP7 expression loss in advanced melanomas, loss of heterozygosity (LOH) was assessed using microsatellite markers encompassing the FABP7 gene. LOH was identified in 10 of 20 (50%) metastatic melanomas at 6q22.31, compared with 0 of 14 primary melanomas (P0.0017). FABP7 as a surrogate biomarker for circulating tumor cells (CTCs) in the blood was assessed by quantitative real-time (qRT)-PCR from melanoma patients blood (n134). Assessment of patients blood showed that FABP7() CTC decreased with disease progression. FABP7 may function as a tumor progression gene and can be used as a potential diagnostic biomarker of early-stage melanoma systemic spreading in blood.

本文言語English
ページ(範囲)221-229
ページ数9
ジャーナルJournal of Investigative Dermatology
130
1
DOI
出版ステータスPublished - 2010 1
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 皮膚病学
  • 細胞生物学

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