Accurate multiplex polymerase chain reaction assay for gender determination from a single cell

T. Hashiba, K. Sueoka, M. Kuroshima, H. Asada, N. Kuji, Y. Yoshimura

研究成果: Article

3 引用 (Scopus)

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For gender determination of preimplantation embryos or circulating fetal cells in maternal blood, we developed a multiplex polymerase chain reaction assay from a single cell. This assay which co-amplifies X (DXZ1)- and Y (DYZ1)-specific repeat sequences, yields a 308-bp band in females and two bands of 154 and 308 bp in males. In a randomized, blinded assay of 100 isolated single amniocytes, 99 (99%) were amplified successfully. All 50 of the XY cells were correctly diagnosed as male (100%), whereas 49 of the 50 XX cells were diagnosed as female (98%). This accurate and efficient assay may be applicable in these clinical settings. Copyright (C) 2000 S. Karger AG, Basel.

元の言語English
ページ(範囲)217-220
ページ数4
ジャーナルGynecologic and Obstetric Investigation
49
発行部数4
DOI
出版物ステータスPublished - 2000 1 1

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ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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