Activated platelets and endothelial cell interaction with neutrophils under flow conditions

S. Mine, T. Fujisaki, M. Suematsu, Y. Tanaka

研究成果: Article査読

35 被引用数 (Scopus)

抄録

Objective. The mechanism of neutrophil adhesion to the endothelium during the earliest stages of acute inflammation, especially before the induction of adhesion molecules on endothelial cells, remains unknown. We studied the possible involvement of platelets in this process. Methods. Neutrophils were added to human umbilical vein-derived endothelial cells (HUVEC) with or without adherent platelets in the presence or absence of adhesion-blocking monoclonal antibodies (mAbs). Adhesion of neutrophils to HUVEC at dynamic flow conditions was assessed using a flow chamber. Results. 1) Thrombin-activated platelets adhered to resting HUVEC at dynamic flow conditions through platelet glycoprotein IIb/IIIa and RGD proteins. 2) Neutrophils tethered to P-selectin induced on thrombin-activated platelets, which were immobilized on HUVEC. 3) Activated neutrophils adhered, via LFA-1, to ICAM-1 on HUVEC. 4) Activated platelets induced interleukin (IL)-8 secretion by HUVEC. Conclusions. Immobilized platelets on the vessel wall with induced P-selectin on the surface biochemically and functionally promote the adhesion of neutrophils to endothelial cells.

本文言語English
ページ(範囲)1085-1092
ページ数8
ジャーナルInternal Medicine
40
11
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • 内科学

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