Activation of a diluted E. coli cell-free transcription-translation system within liposomes by hypertonic concentration

Yukino Matsui, Toshiki Akui, Nobuhide Doi, Kei Fujiwara

研究成果: Article査読

抄録

We present a protocol for activating protein synthesis in liposomes encapsulating a diluted E. coli cell extract-based TX-TL (transcription-translation) system by hypertonic concentration. Protein expression is turned on in the liposome-encapsulated TX-TL system by simple treatment with a concentrated external solution. The expression of sfGFP is demonstrated here, but it can be applied to other proteins. This protocol can be applied to the development of artificial cells utilizing the switch-on mechanism to activate protein expression, responding to the outer environment. For complete details on the use and execution of this protocol, please refer to Akui et al. (2021).

本文言語English
論文番号101003
ジャーナルSTAR Protocols
2
4
DOI
出版ステータスPublished - 2021 12月 17

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 神経科学(全般)
  • 免疫学および微生物学(全般)
  • 医学(全般)

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