TY - JOUR
T1 - ADAMTS-1 cleaves a cartilage proteoglycan, aggrecan
AU - Kuno, Kouji
AU - Okada, Yasunori
AU - Kawashima, Hiroto
AU - Nakamura, Hiroyuki
AU - Miyasaka, Masayuki
AU - Ohno, Hiroshi
AU - Matsushima, Kouji
PY - 2000/8/4
Y1 - 2000/8/4
N2 - A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu1871-Leu1872 bond within the chondroitin sulfate attachment domain of aggrecan. In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo. (C) 2000 Federation of European Biochemical Societies.
AB - A disintegrin-like and metalloproteinase with thrombospondin type I motifs-1 (ADAMTS-1) is an extracellular matrix-anchored metalloproteinase. In this study we have demonstrated that ADAMTS-1 is able to cleave a major cartilage proteoglycan, aggrecan. N-terminal sequencing analysis of the cleavage product revealed that ADAMTS-1 cleaves the Glu1871-Leu1872 bond within the chondroitin sulfate attachment domain of aggrecan. In addition, deletional analysis demonstrated that the C-terminal spacer region of ADAMTS-1 is necessary to degrade aggrecan. These results suggest that ADAMTS-1 may be involved in the turnover of aggrecan in vivo. (C) 2000 Federation of European Biochemical Societies.
KW - Aggrecan
KW - Cartilage degradation
KW - Disintegrin and metalloproteinase thrombospondin
KW - Extracellular matrix
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U2 - 10.1016/S0014-5793(00)01854-8
DO - 10.1016/S0014-5793(00)01854-8
M3 - Article
C2 - 10930576
AN - SCOPUS:0034604613
VL - 478
SP - 241
EP - 245
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -