Adaptive optics with spatio-temporal lock-in detection for temporal focusing microscopy

Tomohiro Ishikawa, Keisuke Isobe, Kenta Inazawa, Kana Namiki, Atsushi Miyawaki, Fumihiko Kannari, Katsumi Midorikawa

研究成果: Article査読

抄録

Wavefront distortion in temporal focusing microscopy (TFM) results in a distorted temporal profile of the excitation pulses owing to spatio-temporal coupling. Since the pulse duration is dramatically changed in the excitation volume, it is difficult to correct the temporal profile for a thick sample. Here, we demonstrate adaptive optics (AO) correction in a thick sample. We apply structured illumination microscopy (SIM) to an AO correction in wide-field TFM to decrease the change in the pulse duration in the signal detection volume. The AO correction with SIM was very successful in a thick sample for which AO correction with TFM failed.

本文言語English
ページ(範囲)29021-29033
ページ数13
ジャーナルOptics Express
29
18
DOI
出版ステータスPublished - 2021 8 30

ASJC Scopus subject areas

  • 原子分子物理学および光学

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