Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy

Junji Tada, Taiki Kono, Akira Suda, Hideaki Mizuno, Atsushi Miyawaki, Katsumi Midorikawa, Fumihiko Kannari

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins.

本文言語English
ページ(範囲)3023-3030
ページ数8
ジャーナルApplied Optics
46
15
DOI
出版ステータスPublished - 2007 5 20

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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