Extracellular adenosine activates P1 receptors (A1, A2A, A2B, A3) on cellular membranes. Here, we investigated the involvement of P1 receptor-mediated signaling in differentiation to regulatory T cells (Treg). Treg were induced in vitro by incubating isolated CD4+CD62L+ naïve murine T cells under Treg-skewing conditions. Antagonists of A1 and A2B receptors suppressed the expression of Foxp3, a specific marker of Treg, and the production of IL-10, suggesting the involvement of A1 and A2B receptors in differentiation to Treg. We also investigated the effect of these antagonists on T cell activation, which is essential for differentiation to Treg, and found that A1 antagonist, but not A2B antagonist, suppressed T cell activation. We conclude that A1 and A2B receptors are both involved in differentiation to Treg, but through different mechanisms. Since A2B antagonist blocked differentiation to Treg without suppressing T cell activation, it is possible that blockade of A2B receptor would facilitate tumor immunity.
|ジャーナル||Biochemical and Biophysical Research Communications|
|出版ステータス||Published - 2011 5月 27|
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