Adhesion of lactobacillus to intestinal mucin

Keita Nishiyama, Takao Mukai

研究成果: Chapter

1 被引用数 (Scopus)

抄録

Adhesion to intestinal mucin is one of the main in vitro tests for the study of probiotic strains. Mucins immobilized on microtiter plates and glass slides are easy and excellent methods used for the quantitative analysis of Lactobacillus adhesion. However, to maintain the performance of the quantitative analysis, these methods must be chosen appropriately according to the nature and characteristics of the strain, such as tolerance to surfactant and the ability to self-aggregate. Here we describe two methodologies used to evaluate adhesion of Lactobacillus to mucin, in addition to the isolation and purification of mucins from porcine colonic tissues.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ159-166
ページ数8
DOI
出版ステータスPublished - 2019
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1887
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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