Aerotaxis and aerokinesis of Dictyostelium discoideum under hypoxic microenvironments

S. Hirose, J. P. Rieu, C. Anjard, O. Cochet-Escartin, H. Kikuchi, K. Funamoto

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

Although spatiotemporal changes of oxygen in a microenvironment are known to affect the cellular dynamics of various eukaryotes, the details are not fully understood. Here, we describe the aerotaxis and aerokinesis of Dictyostelium discoideum (Dd), which has long been employed as a model organism for eukaryotic cells. We developed a microfluidic device capable of time-lapse observation of cultured cells while controlling oxygen concentrations in microchannels. Migratory behaviors of Dd were observed and quantitatively evaluated under an oxygen concentration gradient from 0% to 21% O 2 , as well as in various uniform oxygen conditions. In a hypoxic region within the oxygen concentration gradient, Dd migrated toward regions of higher oxygen concentration at increased velocity, which was independent of cell density. Observed under uniform oxygen concentrations of 1%, 2%, 3%, and 21%, the migration velocity of Dd increased significantly in hypoxic environments of 2% O 2 or less. Thus, Dd shows aerotaxis, directed by the oxygen concentration gradient, and simultaneously shows aerokinesis, changing the migration velocity according to the oxygen concentration itself.

本文言語English
ホスト出版物のタイトル43rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2021
出版社Institute of Electrical and Electronics Engineers Inc.
ページ1187-1190
ページ数4
ISBN(電子版)9781728111797
DOI
出版ステータスPublished - 2021
外部発表はい
イベント43rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2021 - Virtual, Online, Mexico
継続期間: 2021 11月 12021 11月 5

出版物シリーズ

名前Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN(印刷版)1557-170X

Conference

Conference43rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2021
国/地域Mexico
CityVirtual, Online
Period21/11/121/11/5

ASJC Scopus subject areas

  • 信号処理
  • 生体医工学
  • コンピュータ ビジョンおよびパターン認識
  • 健康情報学

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