We have made an attempt to detect bulge DNA by surface plasmon resonance (SPR) biosensor. Peptide nucleic acid (PNA) was conjugated onto polymeric microsphere, which had been prepared by soap-free emulsion polymerization. SPR results indicated that PNA-conjugated microsphere can detect bulge DNA, and amplify SPR signal. Furthermore, we succeeded in magnifying SPR response more than ten times maintaining high discrimination ability by oligolysine-PNA immobilized microsphere.
|出版ステータス||Published - 2006 10月 19|
|イベント||55th SPSJ Annual Meeting - Nagoya, Japan|
継続期間: 2006 5月 24 → 2006 5月 26
|Other||55th SPSJ Annual Meeting|
|Period||06/5/24 → 06/5/26|
ASJC Scopus subject areas