AFM imagings of ferritin molecules bound to LB films of poly-1-benzyl-L-histidine

Satomi Ohnishi, Masahiko Hara, Taiji Furuno, Wolfgang Knoll, Hiroyuki Sasabe

研究成果: Conference contribution

9 被引用数 (Scopus)

抄録

A water-soluble protein, ferritin, on a silicon surface has been imaged in pure water at room temperature with the atomic force microscope (AFM). The sample were prepared by binding ferritin molecules electrostatically to a charged polypeptide layer of poly-1-benzyl-L-histidine (PBLH). The hexagonal arrangement of ferritin molecules was imaged with high reproducibility, since the force between tip and the sample surface could be kept sufficiently lower than 10-10 N. The applied force can be stabilized and weakened mainly due to a 'self-screening effect' of the surface charges of the ferritin-PBLH layer. We demonstrate that the electrostatic-binding sample preparation is one of the suitable methods for soft biological specimens to achieve the nondestructive low-force AFM imagings.

本文言語English
ホスト出版物のタイトルMaterials Research Society Symposium Proceedings
出版社Publ by Materials Research Society
ページ145-150
ページ数6
ISBN(印刷版)1558991905
出版ステータスPublished - 1993
外部発表はい
イベントSymposium on Atomic-scale Imaging of Surfaces and Interfaces -
継続期間: 1992 11月 301992 12月 2

出版物シリーズ

名前Materials Research Society Symposium Proceedings
295
ISSN(印刷版)0272-9172

Other

OtherSymposium on Atomic-scale Imaging of Surfaces and Interfaces
Period92/11/3092/12/2

ASJC Scopus subject areas

  • 材料科学(全般)
  • 凝縮系物理学
  • 材料力学
  • 機械工学

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