Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor receptor: Role of cytoplasmic tail phosphorylation in each process

Isao Ishii, Erika Saito, Takashi Izumi, Michio Ui, Takao Shimizu

研究成果: Article査読

59 被引用数 (Scopus)

抄録

Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor (PAF) receptor were characterized in transfected Chinese hamster ovary cells. Exposure of the cells to PAF led to rapid sequestration of the receptors into the intracellular compartment and desensitization of the response to PAF. The sequestration was inhibited by pretreatments that perturbed the clathrin-mediated pathway. Subsequent removal of PAF by washing with receptor antagonists led to rapid recycling of the sequestered receptors to the cell surface accompanied by resensitization to PAF. To evaluate the potential role of phosphorylation in the receptor cytoplasmic tail during these processes, mutant receptors in which the tails were truncated or substituted, so as to lack serine/threonine residues, were created. PAF phosphorylated the wildtype receptor rapidly and strongly, but the mutants did not. The maximal extent of sequestration of each mutant was lower than that of the wild-type, and one of the substituted mutants showed no sequestration. Furthermore, the sequestration-defective mutant showed evidence of desensitization after agonist stimulation but not resensitization after agonist removal. Thus, agonist-induced phosphorylation of the cytoplasmic tail facilitates but is not essential for receptor sequestration, and sequestration/recycling appears important in receptor resensitization.

本文言語English
ページ(範囲)9878-9885
ページ数8
ジャーナルJournal of Biological Chemistry
273
16
DOI
出版ステータスPublished - 1998 4月 17

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor receptor: Role of cytoplasmic tail phosphorylation in each process」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル