Aldosterone and 18-oxocortisol coaccumulation in aldosterone-producing lesions

Yuki Sugiura, Emi Takeo, Shuichi Shimma, Mai Yokota, Tatsuya Higashi, Tsugio Seki, Yosuke Mizuno, Mototsugu Oya, Takeo Kosaka, Masao Omura, Tetsuo Nishikawa, Makoto Suematsu, Koshiro Nishimoto

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Primary aldosteronism is a secondary hypertensive disease caused by autonomous aldosterone production that often caused by an aldosterone-producing adenoma (APA). Immunohistochemistry of aldosterone synthase (CYP11B2) shows the presence of aldosterone-producing cell clusters (APCCs) even in non-primary aldosteronism adult adrenal cortex. An APCC-like structure also exists as possible APCC-to-APA transitional lesions (a speculative designation) in primary aldosteronism adrenals. However, whether APCCs produce aldosterone or 18-oxocortisol, a potential serum marker of APA, remains unknown because of lack of technology to visualize adrenocorticosteroids on tissue sections. To address this obstacle, in this study, we used highly sensitive Fourier transform ion cyclotron resonance mass spectrometry to image various adrenocorticosteroids, including 18-oxocortisol, in adrenal tissue sections from 8 primary aldosteronism patients with APCC (cases 1-4), possible APCC-to-APA transitional lesions (case 5), and APA (cases 6-8). Further analyses by tandem mass spectrometry imaging allowed us to differentially visualize aldosterone from cortisone, which share identical mass-to-charge ratio value (m/z). In conclusion, these advanced imaging techniques revealed that aldosterone and 18-oxocortisol coaccumulated within CYP11B2-expressing lesions. These imaging outcomes along with a growing body of aldosterone research led us to build a progressive development hypothesis of an aldosterone-producing pathology in the adrenal glands.

本文言語English
ページ(範囲)1345-1354
ページ数10
ジャーナルHypertension
72
6
DOI
出版ステータスPublished - 2018 12

ASJC Scopus subject areas

  • 内科学

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