Alkannin, HSP70 Inducer, Protects against UVB-Induced Apoptosis in Human Keratinocytes

Yoko Yoshihisa, Mariame Ali Hassan, Yukihiro Furusawa, Yoshiaki Tabuchi, Takashi Kondo, Tadamichi Shimizu

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Alkannin is an active constituent from the root extract of Alkanna tinctoria of the Boraginaceae family and it may have utility as a heat shock protein 70 (HSP70) inducer in living organisms. Here, the effects of alkannin-induced HSP70 on ultraviolet (UV) B (40 mJ/cm2)-induced apoptosis were investigated in human keratinocyte HaCaT cells. Pretreatment of cells with alkannin (1 μM) caused significant inhibition of UVB-induced apoptosis and caspase-3 cleavage. On the other hand, the addition of KNK437 (HSP70 inhibitor) reversed the action of alkannin increasing UVB-induced apoptosis in a dose-dependent manner. In addition, differences in gene expression associated with the suppression of UVB-induced apoptosis in the presence of alkannin were investigated using Gene Chip assay. Our results indicate that alkannin suppresses UVB-induced apoptosis through the induction of HSP70 in human keratinocytes, and therefore, we suggest the usefulness of using alkannin as an antiaging agent.

本文言語English
論文番号e47903
ジャーナルPloS one
7
10
DOI
出版ステータスPublished - 2012 10月 22

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

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