Allotype analysis to determine the origin of cytomegalovirus immunoglobulin-G after allogeneic stem cell transplantation

R. Yamazaki, Y. Tanaka, H. Nakasone, M. Sato, K. Terasako-Saito, K. Sakamoto, Y. Akahoshi, H. Nakano, T. Ugai, R. Yamasaki, H. Wada, Y. Ishihara, K. Kawamura, M. Ashizawa, S. I. Kimura, M. Kikuchi, S. Kako, J. Kanda, A. Tanihara, J. NishidaY. Kanda

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Background: Cytomegalovirus (CMV) reactivation still remains a major problem following allogeneic hematopoietic stem cell transplantation (HSCT). Patients and methods: In this study, we analyzed an immunoglobulin allotype, IgG1m(f), in CMV-seropositive HSCT recipients and their donors to distinguish donor-derived antibody from recipient-derived antibody. Eight donor-recipient pairs were informative regarding the appearance of donor-derived immunoglobulin-G (IgG), as the recipients were homozygous null for the IgG1m(f) allotype and the donors were IgG1m(f) positive. In these patients, total IgG, IgM, and allotype-specific IgG against CMV were measured by enzyme-linked immunosorbent assay. All subjects were monitored for at least 9 months after HSCT with (n = 5) or without (n = 3) CMV reactivation. Results: Donor-derived CMV IgG tended to be elevated earlier in patients with CMV-seropositive donors than in those with CMV-seronegative donors. In 1 patient with a CMV-negative donor, donor-derived CMV IgG was not detected until late CMV reactivation. In 3 patients without CMV reactivation, donor-derived CMV IgG was also elevated within 1-6 months after HSCT. Conclusion: In conclusion, the CMV serostatus of the donor may be related to the timing of the appearance of donor-derived CMV IgG and the reconstitution of humoral immunity against CMV, regardless of the CMV antigenemia level after HSCT.

本文言語English
ページ(範囲)904-913
ページ数10
ジャーナルTransplant Infectious Disease
16
6
DOI
出版ステータスPublished - 2014 12 1
外部発表はい

ASJC Scopus subject areas

  • Infectious Diseases
  • Transplantation

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