Allovahlkampfia spelaea Causing Keratitis in Humans

Mohammed Essa Marghany Tolba, Enas Abdelhameed Mahmoud Huseein, Haiam Mohamed Mahmoud Farrag, Hanan El Deek Mohamed, Seiki Kobayashi, Jun Suzuki, Tarek Ahmed Mohamed Ali, Sumio Sugano

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Background: Free-living amoebae are present worldwide. They can survive in different environment causing human diseases in some instances. Acanthamoeba sp. is known for causing sight-threatening keratitis in humans. Free-living amoeba keratitis is more common in developing countries. Amoebae of family Vahlkampfiidae are rarely reported to cause such affections. A new genus, Allovahlkampfia spelaea was recently identified from caves with no data about pathogenicity in humans. We tried to identify the causative free-living amoeba in a case of keratitis in an Egyptian patient using morphological and molecular techniques. Methods: Pathogenic amoebae were culture using monoxenic culture system. Identification through morphological features and 18S ribosomal RNA subunit DNA amplification and sequencing was done. Pathogenicity to laboratory rabbits and ability to produce keratitis were assessed experimentally. Results: Allovahlkampfia spelaea was identified as a cause of human keratitis. Whole sequence of 18S ribosomal subunit DNA was sequenced and assembled. The Egyptian strain was closely related to SK1 strain isolated in Slovenia. The ability to induce keratitis was confirmed using animal model. Conclusions: This the first time to report Allovahlkampfia spelaea as a human pathogen. Combining both molecular and morphological identification is critical to correctly diagnose amoebae causing keratitis in humans. Use of different pairs of primers and sequencing amplified DNA is needed to prevent misdiagnosis.

本文言語English
論文番号e0004841
ジャーナルPLoS Neglected Tropical Diseases
10
7
DOI
出版ステータスPublished - 2016 7月 14

ASJC Scopus subject areas

  • 公衆衛生学、環境および労働衛生
  • 感染症

フィンガープリント

「Allovahlkampfia spelaea Causing Keratitis in Humans」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル