Alteration of cAMP-mediated signal transduction in cerebral ischemia - Binding activity of PKA and phosphorylation of CREB

K. Tanaka, Y. Fukuuchi, S. Nogawa, H. Nozaki, E. Nagata, S. Suzuki, T. Dembo, A. Kosakai

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Binding of cAMP to the regulatory subunit of cAMP-dependent protein kinase (PKA) is an essential step for cAMP-mediated signal transduction including phosphorylation of cAMP response element binding protein (CREB). In the present study, binding activity of PKA with cAMP and CREB phosphorylation were examined in rat focal brain ischemia induced by occlusion of the middle cerebral artery for 1.5 hours followed by various time of recirculation. Binding activity of PKA with cAMP was progressively inhibited during the acute phase of ischemia from the ischemic core to peri-ischemia area. Phosphorylated CREB-positive cells in the ischemic core revealed a significant, but transient increase in number at 3.5 hours of recirculation, followed by a rapid decrease below the control level during the subsequent period. On the other hand, in the peri-ischemia area, the number of phosphorylated CREB-positive cells showed a more marked increase as compared to that in the ischemic core, and the increase continued until 48 hours of recirculation with a tendency for gradual decline. Persistent enhancement of CREB phosphorylation may thus be closely related to the neuronal viability and neuroprotective mechanisms, whereas rapid disappearance of CREB phosphorylation following ischemic insult may clearly precede neuronal death.

本文言語English
ページ(範囲)1298-1299
ページ数2
ジャーナルClinical Neurology
39
12
出版ステータスPublished - 1999 12 1

ASJC Scopus subject areas

  • Clinical Neurology

フィンガープリント 「Alteration of cAMP-mediated signal transduction in cerebral ischemia - Binding activity of PKA and phosphorylation of CREB」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル