Aluminum induces tau aggregation in vitro but not in vivo

Tatsuya Mizoroki, Shunsuke Meshitsuka, Sumihiro Maeda, Miyuki Murayama, Naruhiko Sahara, Akihiko Takashima

研究成果: Article査読

46 被引用数 (Scopus)

抄録

Etiological studies suggest that aluminum (Al) intake might increase an individual's risk of developing Alzheimer's disease (AD). Biochemical analysis data on the effects of Al, however, are inconsistent. Hence, the pathological involvement of Al in AD remains unclear. If Al is involved in AD, then it is reasonable to hypothesize that Al might be involved in the formation of either amyloid plaques or neurofibrillary tangles (NFTs). Here, we investigated whether Al might be involved in NFT formation by using an in vitro tau aggregation paradigm, a tau-overexpressing neuronal cell line (N2a), and a tau-overexpressing mouse model. Although Al induced tau aggregation in a heparin-induced tau assembly assay, these aggregates were neither thioflavin T positive nor did they resemble tau fibrils seen in human AD brains. With cell lysates from stable cell lines overexpressing tau, the accumulation of SDS-insoluble tau increased when the lysates were treated with at least 100 μM Al-maltolate. Yet Al-maltolate caused illness or death in transgenic mice overexpressing human tau and in non-transgenic littermates well before the Al concentration in the brain reached 100 μM. These results indicate that Al has no direct link to AD pathology.

本文言語English
ページ(範囲)419-427
ページ数9
ジャーナルJournal of Alzheimer's Disease
11
4
DOI
出版ステータスPublished - 2007 1 1
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 臨床心理学
  • 老年医学
  • 精神医学および精神衛生

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