Amino acid analysis by capillary electrophoresis-mass spectrometry

Akiyoshi Hirayama, Tomoyoshi Soga

研究成果: Chapter

15 被引用数 (Scopus)

抄録

A method for the determination of underivatized amino acids based on capillary electrophoresis-mass spectrometry is described. To analyze free amino acids simultaneously, a low acidic pH electrolyte was used to confer positive charge on whole amino acids. All protonated amino acids migrated toward the cathode in CE and then were detected by electrospray ionization mass spectrometry with high selectivity and sensitivity. This method is simple, rapid, and selective and could be readily applied to the analysis of free amino acids in various samples.

本文言語English
ホスト出版物のタイトルAmino Acid Analysis
ホスト出版物のサブタイトルMethods and Protocols
編集者Michail Alterman, Peter Hunziker
ページ77-82
ページ数6
DOI
出版ステータスPublished - 2012 1 2

出版物シリーズ

名前Methods in Molecular Biology
828
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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