Amino Acid Analysis by Capillary Electrophoresis-Mass Spectrometry

Akiyoshi Hirayama, Satsuki Ikeda, Asako Sato, Tomoyoshi Soga

研究成果: Chapter

3 被引用数 (Scopus)

抄録

Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ307-313
ページ数7
DOI
出版ステータスPublished - 2019

出版物シリーズ

名前Methods in Molecular Biology
2030
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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