An activity stain for dihydroxy-acid dehydratase

Che Fu Kuo, Tadahiko Mashino, Irwin Fridovich

研究成果: Article査読

3 被引用数 (Scopus)

抄録

An activity stain has been devised for the dihydroxy-acid dehydratase. When applied to polyacrylamide gel electropherograms of crude soluble extracts of Escherichia coli, it detected a single electromorph. The intensity of staining increased with the amount of extract protein applied to the gel. Activity staining demonstrated that (a) anaerobically grown cells contain more extractable dehydratase activity than do aerobically grown cells; (b) exposure of E. coli to 4.2 atm 02 caused virtually complete loss of activity; (c) exposure of cells to paraquat or plumbagin in the presence of dioxygen, but not in its absence, caused a massive loss of activity. These data illustrate the utility of this activity stain and demonstrate that the dehydratase is inactivated by O2- generated within cells.

本文言語English
ページ(範囲)526-530
ページ数5
ジャーナルAnalytical Biochemistry
164
2
DOI
出版ステータスPublished - 1987 8 1
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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