An antibody-free microfluidic paper-based analytical device for the determination of tear fluid lactoferrin by fluorescence sensitization of Tb3+

Kentaro Yamada, Shunsuke Takaki, Nobutoshi Komuro, Koji Suzuki, Daniel Citterio

研究成果: Article査読

83 被引用数 (Scopus)

抄録

An inkjet-printed microfluidic paper-based analytical device (μPAD) for the detection of lactoferrin has been developed. The analyte concentration dependent fluorescence emission, caused by the sensitization of pre-deposited terbium (Tb3+) upon complexation with lactoferrin on the paper device, is captured using a digital camera. The dynamic response range (0.5-3 mg mL−1) and the limit of detection (0.30 mg mL−1) of the μPAD are suitable for the analysis of normal human tears and the detection of eye disorders. Finally, lactoferrin concentrations in human tear samples were analyzed by the μPADs and the assay results corresponded within 6% error to those obtained by an immunoassay (ELISA). The μPADs provide a simple, rapid and accurate method for lactoferrin detection in tear fluid. Results are obtained within 15 min of a single application of 2.5 μL of sample. To the best of our knowledge, this is the first report of a device for lactoferrin quantification relying neither on an immunoassay nor on high cost analytical instrumentation.

本文言語English
ページ(範囲)1637-1643
ページ数7
ジャーナルAnalyst
139
7
DOI
出版ステータスPublished - 2014 3月 3

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 環境化学
  • 分光学
  • 電気化学

フィンガープリント

「An antibody-free microfluidic paper-based analytical device for the determination of tear fluid lactoferrin by fluorescence sensitization of Tb3+」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル