@article{93bc089378b643deaf8cbc7389103f83,
title = "An experimental model for amoebic abscess production in the cheek pouch of the Syrian golden hamster, Mesocricetus auratus",
abstract = "A new experimental model was developed in hamsters for amoebic abscess caused by Entamoeba histolytica. E. histolytica trophozoites were cultured in a liquid axenic medium, and then injected intradermally into the cheek pouch of the Syrian golden hamster, Mesocricetus auratus. Inoculation consistently resulted in abscess formation at the site in 20 of 22 (91%) study animals. The amoebic nature of the abscesses was confirmed by light microscopy and histopathologic examination. Abscess formation was maximal at day 12 post-inoculation. Potential applications of this simple and reliable model include further elucidation of the pathogenesis of invasive amoebiasis, studies of the host response to amoebae, and in vivo evaluation of chemotherapeutic agents that show in vitro efficacy against E. histolytica.",
keywords = "Amoebic abscess, Animal model, Cheek pouch, Entamoeba histolytica, Hamster",
author = "Beg, {M. A.} and S. Kobayashi and Hussainy, {A. S.} and A. Hamada and E. Okuzawa and Smego, {R. A.} and R. Hussain",
note = "Funding Information: This work was financially supported by a visiting research fellowship from the Japanese Overseas Health Administration Centre and the Labor Welfare Corporation (Dr Beg). We would also like to express our sincere gratitude to Prof. T. Takeuchi, Department of Tropical Medicine and Parasitology, Keio University School of Medicine, Tokyo, Japan for permission to work in his laboratory. We also thank Prof. Viqar Zaman for his critical review of the manuscript and useful comments and appreciate the encouragement of Prof. Mushtaq Ahmed.",
year = "2004",
month = sep,
doi = "10.1016/j.parint.2004.02.002",
language = "English",
volume = "53",
pages = "247--254",
journal = "Parasitology International",
issn = "1383-5769",
publisher = "Elsevier Ireland Ltd",
number = "3",
}
