An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid

Li Linxiang, Yoshihiro Abe, Yukiko Nagasawa, Risa Kudo, Noriko Usui, Kazuhiro Imai, Tadahiko Mashino, Masataka Mochizuki, Naoki Miyata

研究成果: Article査読

98 被引用数 (Scopus)

抄録

An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 μmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 μmol/L, where the concentration of Fe(II) was 50 μmol/L. The fluorescence of 2-hydroxy terephthalate was stable at 24 h, and its detection limit by this method was 5 nmol/L (100 fmol).

本文言語English
ページ(範囲)470-474
ページ数5
ジャーナルBiomedical Chromatography
18
7
DOI
出版ステータスPublished - 2004 9月
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 分子生物学
  • 薬理学
  • 創薬
  • 臨床生化学

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